Identification of osmotic stress-responsive genes dependent on HogA and AtfA in Aspergillus nidulans

ABSTRACT: Microarray analysis was used to identify the osmotic stress-responsive genes dependent on HogA and AtfA in the filamentous fungus Aspergillus nidulans. In order to identify such genes, we conducted the several types of experiment. One was a comparison between wild type with and without osmotic shock (Exp.6). Others were comparison between wild type with osmotic shock and each mutant (hogA, Exp.7; atfA, Exp.8) with osmotic shock. Compared the result of Exp.6 with other experiments, we could identify the genes whose expression was induced or repressed in response to osmotic stress in a manner dependent on HogA and AtfA. KEY WORD; Aspergillus nidulans, osmotic stress, HogA, AtfA Conidia of wild type or each mutant were cultured at 37C in 100ml CD medium containing 2% glucose for 18h, and

ORGANISM(S): Emericella nidulans

SUBMITTER: Daisuke Hagiwara 

PROVIDER: E-GEOD-16566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress