Illumina mRNA-Seq of control and hnRNP H knockdown in 293T cells

ABSTRACT: Pre-mRNA splicing is regulated through combinatorial activity of RNA motifs including splice sites and splicing regulatory elements (SREs). Here, we show that the activity of a major class of mammalian SREs is highly sensitive to the strength of the adjacent 5' splice site (5'ss) sequence, and that this has important functional and evolutionary implications. Activity of G-run SREs was higher for intermediate strength 5'ss by ~4-fold relative to weak 5'ss, and by ~1.3-fold relative to strong 5'ss. The dependence on 5'ss strength was supported both by comparative genomics and by microarray and Illumina mRNA-Seq analyses of splicing changes following RNAi against the splicing factor heterogeneous nuclear ribonucleoprotein (hnRNP) H, which binds G-runs. This dependence implies that the res

ORGANISM(S): Homo sapiens

SUBMITTER: Xinshu Xiao 

PROVIDER: E-GEOD-16642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress