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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human late passage hiPSCs (pluripotent stem cells)

ABSTRACT: Detailed analysis comparing induced pluripotent stem cells revealed functional gene expression differences to hESCs that are changed with long term passaging. We used microarrays to detail the changes that are made throughout passaging to hiPSC lines. Experiment Overall Design: hiPSC lines were harvested at long term passages (p50-60) for RNA extraction and hybridization on Affymetrix arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Mark Chin

PROVIDER: E-GEOD-16654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.

Chin Mark H MH Mason Mike J MJ Xie Wei W Volinia Stefano S Singer Mike M Peterson Cory C Ambartsumyan Gayane G Aimiuwu Otaren O Richter Laura L Zhang Jin J Khvorostov Ivan I Ott Vanessa V Grunstein Michael M Lavon Neta N Benvenisty Nissim N Croce Carlo M CM Clark Amander T AT Baxter Tim T Pyle April D AD Teitell Mike A MA Pelegrini Matteo M Plath Kathrin K Lowry William E WE

Cell stem cell 20090701 1

Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain ...[more]

PMID: 19570518

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