Histone H1 binding is restricted by histone variant H3.3 (Expression)

ABSTRACT: Linker histones are involved in the formation of higher-order chromatin structure. Although linker histones have been implicated in the regulation of specific genes, it still remains unclear what their principal binding determinants are and how their repressive function in vitro can be reconciled with presumed broad binding in vivo. We generated a full genome, high resolution binding map of linker histone H1 in Drosophila Kc cells, using DamID. H1 binds at similar levels across much of the genome, both in classical euchromatin and heterochromatin. Strikingly, there are pronounced dips of low H1 occupancy around transcription start sites of active genes and at many distant cis-regulatory sites. H1 dips are not due to lack of nucleosomes. Rather, all regions with low binding of H1 show enri

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ludo Pagie 

PROVIDER: E-GEOD-16884 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress