ORC precisely positions nucleosomes at origins of replication

ABSTRACT: The budding yeast genome is marked by 250-350 origins of DNA replication. These origins are bound by the origin recognition complex (ORC) throughout the cell cycle. ORC has known DNA binding sequence preferences which, though necessary for binding, are not sufficient to fully specify a genomic locus as being bound by ORC, indicating that the cell must use additional chromosomal cues to specify ORC binding sites and origins of replication. Using high-throughput sequencing to precisely locate both ORC binding sites and nucleosome locations genome-wide, we find that a nucleosome depleted region (NDR) and precisely positioned nucleosomes are a ubiquitous feature of yeast replication origins. The ARS consensus sequence (ACS) and adjacent sequences are sufficient to maintain the nucleosome-free

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Matthew Eaton 

PROVIDER: E-GEOD-16926 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress