Paralog Specificity in cDNA Microarray Experiments

ABSTRACT: cDNA microarrays have become the technology of choice for profiling the expression of thousands of genes simultaneously in a single sample. However, cross-hybridization between targets and probes that do not correspond must be considered carefully during the design, analysis, and interpretation of these experiments. Here, we examined this potentially confounding issue by competitively hybridizing fragments with similar sequence obtained from two paralogous genes to a custom cDNA microarray that contains probes for only one of these two genes. We found that although a large contiguous portion of these two fragments was 84% identical and only one target was represented on the array, there was very little cross-hybridization of the non-represented target to either the paralogous probes or oth

ORGANISM(S): Haplochromis burtoni

SUBMITTER: Hans Hofmann 

PROVIDER: E-GEOD-16939 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress