A putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs

ABSTRACT: Identification of a putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs. An experimental purification in which a presumed RNA binding protein was used for an RNA immunoprecipitation and microarray analysis (RIP-CHIP). Total cell extracts were harvested from the conditionally immortalized mouse MPC-5 podocyte cell line 14 days after transfer from 33 to 37 degrees Celsius. The MPC-5 cell lines were stably expressing the Wilm's Tumor protein (+KTS) isoform carrying a modified TAP tag. A fraction of each extract (Input samples) was saved and the remaining extract was applied to a sepharose IgG column, washed, and eluates were harvested after addition of TEV protease and elution in microbiospin columns. Four input and four eluate s

ORGANISM(S): Mus musculus

SUBMITTER: Russ Carstens 

PROVIDER: E-GEOD-16942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress