Gene expression profiling of retinoblastoma Y79 cell line post EpCAM silencing using siRNA

ABSTRACT: Retinoblastoma Y79 cell line was treated with specific siRNA to silence EpCAM gene expression in vitro and RNA was isolated for microarray experiment to study the changes in the whole gene expression profiling in the Y79 cell line. The study identified 465 up-regulated genes (>1.0 fold) and 205 down-regulated genes (<0.5 fold) in response to knockdown of Ep-CAM. Organism used: Homo Sapiens Slides: Whole genome Human 4x44k array Starting material: Cells in RNA Later RNA Samples used: Control, siRNA1 Labeling kit: Agilent Low Input RNA Linear amplification kit Labeling Method: T7 promoter based-linear amplification to generate labelled complementary RNA Total RNA and cRNA Purification Kit: Qiagen’s RNeasy minikit Hybridization Kit: Agilent Insitu Hybridization Kit H

ORGANISM(S): Homo sapiens

SUBMITTER: Sugandan Sivamani 

PROVIDER: E-GEOD-16991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress