High-throughput pyrosequencing of plant virus small RNAs

ABSTRACT: Plant virus infection involves the production of viral small RNAs (vsRNAs) with the potential to associate with distinct Argonaute (AGO)-containing silencing complexes and mediate diverse silencing effects on RNA and chromatin. We used multiplexed, high-throughput pyrosequencing to profile populations of vsRNAs from plants infected with viruses from different genera. Sense and antisense vsRNAs of 20 to 24 nucleotides (nts) spread throughout the entire viral genomes in an overlapping configuration; virtually all genomic nucleotide positions were represented in the dataset. We present evidence to suggest that every genomic position could be a putative cleavage site for vsRNA formation, although viral genomes contain specific regions that serve as preferential sources of vsRNA production. Hot

ORGANISM(S): Melon necrotic spot virus

SUBMITTER: Yu Wang 

PROVIDER: E-GEOD-16996 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress