Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiles of normal human mammary cell subpopulations FACS sorted based on expression of CD49f and EpCAM

ABSTRACT: To delineate epithelial subpopulations in human mammary tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from reduction mammoplasties by fluorescence-activated cell sorting. The resultant Lin- population was fractionated into four distinct subpopulations using CD49f (α6-integrin) and epithelial cell adhesion molecule (EpCAM; also referred to as CD326 and ESA). Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as fibroblast-enriched stromal (CD49f -EpCAM-), mammary stem cell (MaSC)-enriched (CD49f hiEpCAM-), luminal progenitor (CD49f +EpCAM+), and mature luminal (CD49f –EpCAM+) cell subpopulations. Microarray profiling was used to derive gene expression signatures representative of these subpopulations using freshly sorted cells (>90% purity) from normal breast tissue. The four mammary cell subpopulations were found to have distinct gene expression profiles. Four mammary cell subpopulations from three individual patient samples were analyzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Gordon Smyth 

PROVIDER: E-GEOD-16997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Basal-like breast cancers arising in women carrying mutations in the BRCA1 gene, encoding the tumor suppressor protein BRCA1, are thought to develop from the mammary stem cell. To explore early cellular changes that occur in BRCA1 mutation carriers, we have prospectively isolated distinct epithelial subpopulations from normal mammary tissue and preneoplastic specimens from individuals heterozygous for a BRCA1 mutation. We describe three epithelial subsets including basal stem/progenitor, luminal  ...[more]

Similar Datasets

Unknown Gene expression profiles of normal human mammary cell subpopulations FACS sorted based on expression of CD49f and EpCAM
2009-07-09 | GSE16997 | GEO
Transcriptomics A stemness-related ZEB1-MSRB3 axis governs cellular pliancy and cancer genome stability.
2017-03-23 | E-MTAB-4145 | biostudies-arrayexpress
Unknown Gene expression profiles of epithelial and mesenchymal cell populations extracted from normal human breast tissue
2015-09-26 | GSE51200 | GEO
Transcriptomics Genome Wide Expression of Mammary Epithelial Populations in Slug (SNAI2) WT vs Slug Null Mice
2014-03-08 | E-GEOD-55620 | biostudies-arrayexpress
Transcriptomics Elucidating the gene expression profiles of human mammary subpopulations
2012-10-11 | E-GEOD-35399 | biostudies-arrayexpress
Transcriptomics Progesterone and estrogen receptors segregate into different cell subpopulations in the normal human breast
2012-08-17 | E-GEOD-31429 | biostudies-arrayexpress
Transcriptomics Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers
2010-03-20 | E-GEOD-19446 | biostudies-arrayexpress
Unknown Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers
2010-03-21 | GSE19446 | GEO
Unknown Progesterone and estrogen receptors segregate into different cell subpopulations in the normal human breast
2012-08-17 | GSE31429 | GEO
Transcriptomics Transcription profiling of mouse mammary cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen
2007-11-20 | E-MEXP-423 | biostudies-arrayexpress