Gene expression profiling of retinoblastoma Y79 cell line treated with scopoletin from Morinda citrifolia in vitro

ABSTRACT: Retinoblastoma Y79 cell line was treated with scopoletin in vitro and RNA was isolated for microarray experiment to study the changes in the whole gene expression profiling in the Y79 cell line. The study identified 1675 up-regulated genes (>1.0 fold) and 1879 down-regulated genes (<-1 fold) in response to scopoletin treatment Slides:Whole genome Human 4x44k array Starting material: Cells in RNA Later RNA Samples used: Y79_c_Noni Extract,Control Labeling kit: Agilent’s Quick-Amp labeling Kit Labeling Method: T7 promoter based-linear amplification to generate labeled complementary RNA Total RNA and cRNA Purification Kit: Qiagen’s RNeasy minikit Cat Hybridization Kit: Agilent’s In situ Hybridzation kit Hybridization protocol The fragmented cRNA were mixed with 25

ORGANISM(S): Homo sapiens

SUBMITTER: Sugandan Sivamani 

PROVIDER: E-GEOD-16998 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress