Gene expression analysis study in cerulenin treated and control Y79 cells

ABSTRACT: Gene expression analysis study in cerulenin treated - 7µg/ml cerulenin treated Y79 cells and control Y79 cells Organism used: Homo sapiens Slides: Gene Expression Whole Human Genome 4x44k Starting material: Cells in RNA later RNA Samples used: :Control Y79 and Treated_Cerulenin Labeling kit: Agilents Low input RNA linear amplification Kit one color Labeling Method: T7 promoter based-linear amplification to generate labeled complementary RNA Total RNA and cRNA Purification Kit: Qiagen’s RNeasy minikit Hybridization Kit: Agilent’s In situ Hybridzation kit Hybridization protocol The fragmented cRNA were mixed with 25ul of 2x GE Hybridization Buffer (Agilent). About 45ul of the resulting mixture was applied to the Microarray and hybridized at 65degC for 17 hours in

ORGANISM(S): Homo sapiens

SUBMITTER: Subramaniam Krishnakumar 

PROVIDER: E-GEOD-16999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress