High definition profiling of mammalian DNA methylation by array capture and single molecule bisulfite sequencing

ABSTRACT: DNA methylation stabilizes developmentally programmed gene expression states. Aberrant methylation is associated with disease progression and is a common feature of cancer genomes. Presently, few methods enable quantitative, large-scale, single-base resolution mapping of DNA methylation states in desired regions of a complex mammalian genome. Here, we present an approach that combines array-based hybrid selection and massively parallel bisulfite sequencing to profile DNA methylation in genomic regions spanning hundreds of thousands of bases. This single molecule strategy enables methylation variable positions to be quantitatively examined with high sampling precision. Using bisulfite capture, we assessed methylation patterns across 324 randomly selected CpG islands (CGI) representing more

ORGANISM(S): Homo sapiens

SUBMITTER: Emily Hodges 

PROVIDER: E-GEOD-17001 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress