Reorganization of nuclear lamina – genome interactions upon differentiation of embryonic stem cells.

ABSTRACT: The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. We present a genome-wide analysis of the interactions between chromatin and the nuclear lamina during differentiation of mouse embryonic stem cells (ESCs) into lineage-committed neural precursor cells (NPCs) and terminally differentiated astrocytes. Chromatin in each of these cell types shows a similar organization into large lamina associated domains (LADs), which represent a transcriptionally repressive environment. During sequential differentiation steps, lamina interactions are progressively modified at hundreds of genomic locations. This remodeling is typically confined to individual transcription units and involves many genes that determine cellular identi

ORGANISM(S): Mus musculus

SUBMITTER: Bas van Steensel 

PROVIDER: E-GEOD-17051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress