Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Identification of direct transcriptional targets of V600EBRAF/MEK in melanoma

ABSTRACT: A375P melanoma cells were treated with 1uM of the MEK inhibitor PD184352 or 0.4uM of the V600EBRAF inhibitor PLX4720 for 2hr, 6hr and 24hrs. DMSO treatment for 2hr, 6hr and 24hrs serves as the negative control Triplicate experiments were performed for DMSO, PD184352 and PLX4720 treatment at 3 timepoints - 2, 6, 24hrs.

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Marais

PROVIDER: E-GEOD-17089 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of direct transcriptional targets of (V600E)BRAF/MEK signalling in melanoma.

Packer Leisl M LM East Philip P Reis-Filho Jorge S JS Marais Richard R

Pigment cell & melanoma research 20090804 6

Oncogenic mutations in BRAF are common in melanoma and drive constitutive activation of the MEK/ERK pathway. To elucidate the transcriptional events downstream of (V600E)BRAF/MEK signalling we performed gene expression profiling of A375 melanoma cells treated with potent and selective inhibitors of (V600E)BRAF and MEK (PLX4720 and PD184352 respectively). Using a stringent Bayesian approach, we identified 69 transcripts that appear to be direct transcriptional targets of this pathway and whose ex ...[more]

PMID: 19682280

Dataset Information

Identification of direct transcriptional targets of V600EBRAF/MEK in melanoma

Publications

Identification of direct transcriptional targets of (V600E)BRAF/MEK signalling in melanoma.

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