Transcription profiling of activated or unactivated T cells isolated from peripheral human leukocytes under gravity or vectorless gravity conditions

ABSTRACT: Peripheral blood leukocytes of three human donors (Donors 2, 4, and 5) were isolated from blood bank buffy-coat preparations by Ficoll-Hypaque density gradient centrifugation, and T-cells were purified with high affinity negative selection human CD3+ T-cell enrichment columns. The cells were resuspended in RPMI-1640 with 10% FCS with approximately 3-8 millions cells/mL at room temperature overnight. T-cells were then either unactivated (1g 0 hr), activated (1g 4 hr) with a final concentration of 5 mg/mL Con A + 4 mg/mL anti-CD28 antibody and incubated for 4 hours at 37°C, or loaded onto a RPM rotating at 60°/s (vg). T-cells loaded on the RPM were cultured for 2 hours prior to collection or activation to allow equilibration of cells to the new environment. After incubation, 1 mL

ORGANISM(S): Homo sapiens

SUBMITTER: Boonyaratanakornkit Jim, 

PROVIDER: E-GEOD-1709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress