Illumina sequencing of small RNAs from C. elegans embryos

ABSTRACT: Caenorhabditis elegans is one of the most prominent model systems to study embryogenesis. However, it has been impractical to collect large amounts of precisely staged embryos. Thus, early C. elegans embryogenesis has not been amenable to most modern high-throughput genomics or biochemistry assays. To overcome this problem, we devised a method to collect large amounts of cleanly staged C. elegans embryos by Fluorescent Activated Cell Sorting (termed eFACS). eFACS can in principle be applied to all embryonic developmental stages up to hatching. As a proof of principle we show that a single eFACS run routinely yields tens of thousands of almost perfectly staged one-cell embryos. Since in animals the earliest embryonic events are driven by post-transcriptional regulation, we combined eFACS wi

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Jonas Maaskola 

PROVIDER: E-GEOD-17153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress