Structural and Functional Analysis of Viral siRNAs using 454 sequencing

ABSTRACT: We profiled vsiRNAs using two different high-throughput sequencing platforms and also developed a hybridisation-based array approach. The profiles obtained through the Solexa platform and by hybridisation were very similar to each other but different from the 454 profile. Both deep sequencing techniques revealed a strong bias in vsiRNAs for the positive strand of the virus and identified regions on the viral genome that produced vsiRNA in much higher abundance than other regions. The hybridisation approach also showed that the position of highly abundant vsiRNAs were the same in different plant species and in the absence of RDR6. We used the TerminatorTM 5'-Phosphate-Dependent Exonuclease to study the 5' end of vsiRNAs and showed that a perfect control duplex was not digested by the enzyme

ORGANISM(S): Nicotiana benthamiana

SUBMITTER: Simon Moxon 

PROVIDER: E-GEOD-17164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress