Transcription profiling of Arabidopsis seedlings treated with chitosan
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ABSTRACT: We treated Arabidopsis seedlings with chitosan and carried out a transcript profiling analysis (GeneChip microarrays) in order to identify genes and transcription factors regulated by chitosan. The results showed that jasmonate and defense responsive genes, camalexin and lignin biosynthetic genes were among genes up-regulated by chitosan. Several transcription factors are also strongly induced by chitosan. The results suggested that chitosan can be used as a strong elicitor of defense pathways. Experiment Overall Design: Arabidopsis thaliana, ecotype Columbia (Col-0) seeds were sterilized for 7 min in 1.7% (v/v) bleach solution, incubated over night in 4% PPM (Plant Preservative Mixture, Plant Cell Technology, Washington DC, USA) in a full strength sterilized Murashige-Skoog (MS) salt solu
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Pierdomenico Perata
PROVIDER: E-GEOD-17193 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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