ChIP to AIRE regulated genes in HEK 293 cells

ABSTRACT: The Autoimmune Regulator (AIRE) protein is expressed in thymic medullary epithelial cells, where it promotes the ectopic expression of tissue-restricted antigens needed for efficient negative selection of developing thymocytes. Mutations in AIRE cause APECED syndrome, which is characterized by a breakdown of self-tolerance. The molecular mechanism by which AIRE increases the expression of a variety of different genes remains unknown. Here, we studied AIRE-regulated genes using whole genome expression analysis and chromatin immunoprecipitation. We show that AIRE preferentially activates genes that are tissue-specific and characterized by low levels of initial expression in stably transfected HEK293 cell model and mouse thymic medullary epithelial cells. In addition, the AIRE-regulated genes

ORGANISM(S): Homo sapiens

SUBMITTER: Pärt Peterson 

PROVIDER: E-GEOD-17216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress