A tomato fruit RNA extraction method that isolates mRNAs encoding secreted and endomembrane associated proteins

ABSTRACT: Background: During the analysis of ripening related gene expression in tomato fruit, we observed a bias towards certain classes of messenger RNA based upon the type of buffer used in the initial step of the extraction procedure. We postulated that there was a functional association of the separated transcripts. To test this hypothesis, we carried out extractions from the same tissue sample where only the buffer varied. Transcripts were hybridised to Affymetrix oligo arrays and the data was analysed according to the predicted cellular component of the encoded proteins. Results: The use of an extraction buffer that lacked high levels of caeotropic agents resulted in a reduction of mRNAs encoding proteins that were either secreted or were predicted to be routed through the endomembrane syst

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Mervin Poole 

PROVIDER: E-GEOD-17222 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress