Transcription profiling of human primary hepatocytes treated with transcription factor PPAR agonist Wy14643

ABSTRACT: Studies in mice have shown that PPARalpha is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARalpha in human liver. Here we set out to compare the function of PPARalpha in mouse and human hepatocytes via analysis of target gene regulation. Primary hepatocytes from 6 human and 6 mouse donors were treated with PPARalpha agonist Wy14643 and gene expression profiling was performed using Affymetrix GeneChips followed by a systems biology analysis. Baseline PPARalpha expression was similar in human and mouse hepatocytes. Depending on species and time of exposure, Wy14643 significantly induced the expression of 362-672 genes. Surprisingly minor overlap was observed between the Wy14643-regulated genes from

ORGANISM(S): Homo sapiens

SUBMITTER: Guido Hooiveld 

PROVIDER: E-GEOD-17251 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress