Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data comparing human foreskin keratinocytes to epithelial derivatives of human embryonic stem cells (H9)

ABSTRACT: Whole genome expression array comparison of primary keratinocytes to retinoic acid-differentiated hES cell derivatives Cells were differentiated according to Metallo et al. Stem Cells 2008 A 3 chip study was conducted using competitive hybridization comparing pFKs to hEKs obtained in 3 independent differentiation experiments

ORGANISM(S): Homo sapiens

SUBMITTER: Christian Metallo

PROVIDER: E-GEOD-17265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.

Metallo Christian M CM Azarin Samira M SM Moses Laurel E LE Ji Lin L de Pablo Juan J JJ Palecek Sean P SP

Tissue engineering. Part A 20100101 1

Human embryonic stem (hES) cells are an attractive source of cellular material for scientific, diagnostic, and potential therapeutic applications. Protocols are now available to direct hES cell differentiation to specific lineages at high purity under relatively defined conditions; however, researchers must establish the functional similarity of hES cell derivatives and associated primary cell types to validate their utility. Using retinoic acid to initiate differentiation, we generated high-pur ...[more]

PMID: 19686061

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Project description:The biological effects of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) on osteoblast differentiation and function differ significantly depending upon the cellular state of maturation. To explore this phenomenon mechanistically, we examined the impact of 1,25(OH)2D3 on the transcriptomes of both pre-osteoblastic (POBs) and differentiated osteoblastic (OBs) MC3T3-E1 cells, and assessed localization of the vitamin D receptor (VDR) at sites of action on a genome-scale using ChIP-seq analysis. We observed that the 1,25(OH)2D3-induced transcriptomes of POBs and OBs were quantitatively and qualitatively different, supporting not only the altered biology observed but the potential for a change in VDR interaction at the genome as well. This idea was confirmed through discovery that VDR cistromes in POBs and OBs were also strikingly different. Depletion of VDR binding sites in OBs, due in part to reduced VDR expression, was the likely cause of the loss of VDR-target gene interaction. Continued novel regulation by 1,25(OH)2D3, however, suggested that factors in addition to the VDR might also be involved. Accordingly, we show that transcriptomic modifications are also accompanied by changes in genome binding of the master osteoblast regulator RUNX2 and the chromatin remodeler C/EBPβ. Importantly, genome occupancy was also highlighted by the presence of epigenetic enhancer signatures which were selectively changed in response to both differentiation and 1,25(OH)2D3. The impact of VDR, RUNX2, and C/EBPβ on osteoblast differentiation is exemplified by their actions at the Runx2 and Sp7 gene loci. We conclude that each of these mechanisms may contribute to the diverse actions of 1,25(OH)2D3 on differentiating osteoblasts. RNA was isolated and applied to gene expression microarrays in undifferentiated MC3T3-E1 cells as well as post 15 day osteogenic differentiation MC3T3-E1 cells, which were treated for 24 hours with 10-7M 1,25(OH)2D3. For the vehicle matched samples, please refer to study GSE41955. The samples were completed in biological triplicate.

Dataset Information

Expression data comparing human foreskin keratinocytes to epithelial derivatives of human embryonic stem cells (H9)

Publications

Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.

Similar Datasets

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