Partitioning the C. elegans genome by nucleosome modification, occupancy, and positioning

ABSTRACT: We have characterized two post-translational histone modifications in C. elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional and coverage maps. Two methylated histone H3 populations were chosen for comparison: H3K4 histone methylation (associated with active chromosomal regions) and H3K9 histone methylation (associated with inactivity). From analysis of the sequence data, we found nucleosome cores with these modifications to be enriched in two distinct partitions of the genome; H3K4 methylation was particularly prevalent in promoter regions of widely-expressed genes while H3K9 methylation was enriched on sp

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Sam Gu 

PROVIDER: E-GEOD-17284 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress