Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling

ABSTRACT: We examined early and late gene expression changes using the IT LPS model of Acute Lung Injury (ALI). In this model, injury peaks at day 4 and is almost completely resolved by day 10 in wild type (WT) C57BL/6 mice. In contrast to the pattern in WT mice, lymphocyte-deficient Rag-1 -/- mice exhibit strikingly delayed resolution despite similar initial injury. Total RNA was isolated from mouse lung at time 0, 1, 4, and 10 days following LPS treatment of wild type and Rag-1 null mice using TRIzol reagent (Invitrogen Life Technologies), then purified using RNAeasy columns (Qiagen) with DNase I treatment according to the manufacturerâ??s recommendations. The quality of RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies).

ORGANISM(S): Mus musculus

SUBMITTER: Neil Aggarwal

PROVIDER: E-GEOD-17355 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulatory T cell-mediated resolution of lung injury: identification of potential target genes via expression profiling.

Aggarwal Neil R NR D'Alessio Franco R FR Tsushima Kenji K Sidhaye Venkataramana K VK Cheadle Christopher C Grigoryev Dmitry N DN Barnes Kathleen C KC King Landon S LS

Physiological genomics 20091222 2

In animal models of acute lung injury (ALI), gene expression studies have focused on the acute phase of illness, with little emphasis on resolution. In this study, the acute phase of intratracheal lipopolysaccharide (IT LPS)-induced lung injury was similar in wild-type (WT) and recombinase-activating gene-1-deficient (Rag-1(-/-)) lymphocyte-deficient mice, but resolution was impaired and resolution-phase lung gene expression remained different from baseline only in Rag-1(-/-) mice. By focusing o ...[more]

PMID: 20028937

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Dataset Information

Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling

Publications

Regulatory T cell-mediated resolution of lung injury: identification of potential target genes via expression profiling.

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