Design and performance of a turbot (Scophthalmus maximus) oligo-microarray based on ESTs from immune tissues

ABSTRACT: An EST database from immune tissues was used to design the first high density turbot (Scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. Specific oligonucleotides (60mers) were successfully designed for 2716 out of 3482 unique sequences of the database. The performance of the microarray and the sources of variation along microarray analysis were examined on spleen pools of controls and Aeromonas salmonicida challenged fish at 3 days post-infection. An asymmetric hierarchical design was employed to ascertain the noise associated with biological and technical (RNA extraction, labeling, hybridization, slide and dye bias) factors using one-colour (1C) and two-colour (2C) -labeling approaches. The high correlation coefficient between r

ORGANISM(S): Psetta maxima

SUBMITTER: Adrian Perez 

PROVIDER: E-GEOD-17357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress