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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Innervated and 2 month Denervated Rat EDL muscles

ABSTRACT: The right legs of 8 Brown-Norway male rats were denervated by a high sciatic nerve section in the hip region of the hind limb.Two months after denervation (6 months of age), extensor digitorum longus (EDL) muscles were removed from the operated legs. The EDL muscles from 8 age-matched non-operated rats served as innervated controls. Total RNA was isolated, labeled cDNA was prepared and hybridized to the Rat Atlas 1.2 Array II membranes (Clontech Laboratories, Palo Alto, CA).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Tatiana Kostrominova

PROVIDER: E-GEOD-1741 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.

Kostrominova Tatiana Y TY Dow Douglas E DE Dennis Robert G RG Miller Richard A RA Faulkner John A JA

Physiological genomics 20050419 2

Loss of innervation in skeletal muscles leads to degeneration, atrophy, and loss of force. These dramatic changes are reflected in modifications of the mRNA expression of a large number of genes. Our goal was to clarify the broad spectrum of molecular events associated with long-term denervation of skeletal muscles. A microarray study compared gene expression profiles of 2-mo denervated and control extensor digitorum longus (EDL) muscles from 6-mo-old rats. The study identified 121 genes with in ...[more]

PMID: 15840640

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Innervated and 2 month Denervated Rat EDL muscles

Project description:The right legs of 8 Brown-Norway male rats were denervated by a high sciatic nerve section in the hip region of the hind limb.Two months after denervation (6 months of age), extensor digitorum longus (EDL) muscles were removed from the operated legs. The EDL muscles from 8 age-matched non-operated rats served as innervated controls. Total RNA was isolated, labeled cDNA was prepared and hybridized to the Rat Atlas 1.2 Array II membranes (Clontech Laboratories, Palo Alto, CA). Keywords: other

2004-09-10 | GSE1741 | GEO

Myasthenia gravis model: gene expression profile in diaphragm, extensor digitorum longus and extraocular muscles of rats

Project description:gene expression profile in diaphragm (DIA), extensor digitorum longus (EDL) and extraocular (EOM) muscles of rats with actively induced experimentally acquired MG (EAMG) using Affymetrix rat RAE230 gene chip. Keywords: myasthenia gravis (MG), rats with actively induced experimentally acquired MG (EAMG)

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Gene expression profile in mouse skeletal muscle after 4F induction

Project description:Analysis of soleus (SOL) and extensor digitorum longus (EDL) muscles isolated from Acta1-Cre+/4Fhet (as treatment) and Acta1-Cre-/4Fhet (as control) mice. Results provide unbiased gene expression profile of SOL and EDL muscles after 4F induction.

2021-03-22 | GSE148911 | GEO

Expression data from skeletal muscles of Ts1Cje and disomic C57BL/6 mice

Project description:To determine the gene expression profile of extensor digitorum longus (EDL) and soleus (SO) muscles of wild-type and Ts1Cje mouse model of Down Syndrome (DS). Two types of skeletal muscles (EDL and SO) were harvested from both Ts1Cje and its disomic littermate.

2018-09-06 | GSE96572 | GEO

Comparative Transcriptome Analysis between SOL and EDL Muscle in Yorkshire pigs using Affymetrix microarray

Project description:Skeletal muscle is heterogeneous in nature and distinguished as red muscle and white muscle because of their myofiber composition. Soleus (SOL) is a typical red muscle and extensor digitorum longus (EDL) is a typical white muscle. In this study, we compared the transcriptome difference of soleus and extensor digitorum longus from three 10-week-old Yorkshire boars with porcine Affymetrix microarray.

2014-12-31 | E-MTAB-2419 | biostudies-arrayexpress

miRNA expression in fast and slow skeletal muscle of wild type and SOD1-G93A mice

Project description:Amyotrophic lateral sclerosis (ALS) is a lethal motor neuron disease that progressively debilitates neuronal cells that control voluntary muscle activity. In a mouse model of ALS that expresses mutated human superoxide dismutase 1 (SOD1-G93A) skeletal muscle is one of the tissues affected early by mutant SOD1 toxicity. Fast-twitch and slow-twitch muscles are differentially affected in ALS patients and in the SOD1-G93A model, fast-twitch muscles being more vulnerable. We used miRNA microarrays to investigate miRNA alterations in fast-twitch (EDL) and slow-twitch (soleus) skeletal muscles of symptomatic SOD1-G93A animals and their age-matched wild type littermates. At age of 90 days RNA was extracted from extensor digitorum longus (EDL) and soleus (SOL) muscles of male SOD1-G93A animals and their age-matched wild type male littermates. RNA was hybridized on Affymetrix Multispecies miRNA-2_0 Array.

2014-03-04 | E-GEOD-55507 | biostudies-arrayexpress

Skeletal muscle myonuclei-specific ChIP-Seq of H3K4me3 and H3K27ac

Project description:Skeletal muscle is a heterogeneous tissue. To study the myo-specific epigenetic landscape in the slow soleus and fast extensor digitorum longus (EDL) muscle, myonuclei were purified with PCM1 -an endogenous mark specific for mature myonuclei. Chromatin immunoprecipitation sequencing (ChIP-Seq) was performed using antibodies against the histone marks H3K4me3 and H3K27ac. Comparison of the differently enriched areas between the two muscles shows that the epigenetic landscape reflects the functional properties of the muscles, each with a distinct regulatory program involving distal regulatory enhancers.

2021-10-22 | GSE182667 | GEO

Transcriptomic analysis of mdx mouse muscles reveals a signature of early human Duchenne muscular dystrophy

Project description:The mdx mouse (C57BL/10ScSn-DMDmdx/J), like Duchenne muscular dystrophy (DMD) patients, lacks the protein dystrophin. However, the mdx mouse has a normal lifespan and mild pathology while DMD remains a severe, fatal disease. New mouse models have been developed that are more severely affected but they have not replaced the mdx mouse in DMD research. A few years ago RNA-sequencing (RNA-seq) results of biopsies from DMD and normal human muscle were published but we could not find equivalent data for the mouse. We now report RNA-sequencing of three wild-type and mdx mouse muscles: the flexor digitorum brevis (FDB), the extensor digitorum longus (EDL), and the soleus (SOL). The FDB, a plantar foot muscle is often used for in vivo and ex vivo experiments but may be less affected by the lack of dystrophy than the hindlimb muscles. We compared muscles from 2- and 5-month old mice to investigate the time-course of the mdx pathology. The results show a muscle- and age-dependent parallel between mdx and DMD muscles. Although the FDB is less affected than EDL and SOL at 2 months, the three muscles show, at both ages, activation of close to 100 genes from 7 pathways that are affected in presymptomatic DMD patients and have been called "DMD disease signature".

2021-06-22 | GSE162455 | GEO

Transcriptional responses to chronic oxidative stress in skeletal muscle

Project description:We measured gene expression differences in the extensor digitorum longus (edl) skeletal muscle between wild-type and mice lacking Cu, Zn-superoxide dismutase to determine the effect of chronic oxidative stress on skeletal muscle. We analyzed 4 edl samples from WT mice and 4 samples from the SOD1 knockouts.

2011-04-26 | E-GEOD-28837 | biostudies-arrayexpress

Transcriptional responses to chronic oxidative stress in skeletal muscle

2011-04-26 | GSE28837 | GEO

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