Gene regulation in Bacillus subtilis by the compatible solute glycine betaine

ABSTRACT: Glycine betaine (GB) is a potent osmoprotectant for salt stressed Bacillus subtilis cells, which possess three high-affinity uptake systems for GB. OpuA is the dominant transporter for this compatible solute. Northern blot analysis, primer extension experiments and opuA-treA reporter gene fusion studies demonstrated that opuA expression is strongly induced at high osmolality from a single SigA-type promoter. Promoter mutations that improve the match of the opuA promoter to the consensus sequence substantially increase basal-level expression but reduce inducibility by high salinity. Expression of opuA is sensitively controlled by GB, which causes significant repression of opuA transcription at low and high salinity. GB influences the kinetics as well as the final level of high salinity indu

ORGANISM(S): Bacillus subtilis

SUBMITTER: Annette Wensing 

PROVIDER: E-GEOD-17435 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress