Unknown,Transcriptomics,Genomics,Proteomics

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Drosophila elevated CO2 (hypercapnia) microarray

ABSTRACT: The effects of elevated CO2 (hypercapnia) on organisms are not well known, nor are the molecular pathways by which organisms sense CO2. We have performed microarray analysis on Drosophila in order to develop a genetic model for better understanding the effects of CO2. Equal numbers of 5-day old male and female flies (at least 40 total) were exposed to 13% CO2 or air for 24h and RNA extracted. Gene expression changes in CO2 compared with air were analyzed. Experiments were performed in triplicate and the 3 separate microarray experiments are included.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Taneli Helenius 

PROVIDER: E-GEOD-17444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection.

Helenius Iiro Taneli IT   Krupinski Thomas T   Turnbull Douglas W DW   Gruenbaum Yosef Y   Silverman Neal N   Johnson Eric A EA   Sporn Peter H S PH   Sznajder Jacob I JI   Beitel Greg J GJ  

Proceedings of the National Academy of Sciences of the United States of America 20091021 44

Elevated CO(2) levels (hypercapnia) frequently occur in patients with obstructive pulmonary diseases and are associated with increased mortality. However, the effects of hypercapnia on non-neuronal tissues and the mechanisms that mediate these effects are largely unknown. Here, we develop Drosophila as a genetically tractable model for defining non-neuronal CO(2) responses and response pathways. We show that hypercapnia significantly impairs embryonic morphogenesis, egg laying, and egg hatching  ...[more]