Identification of transcription factor HLH-8 binding sites in C.elegans

ABSTRACT: We, being members of modENCODE consortium, have established an experimetal pipeline in C.elegans that allows global identification of the binding sites for transcription factors using chromatin immunoprecipitation followed by illumina high-throughput sequencing (ChIP-seq). In current study, we identified the binding sites for HLH-8 at L3 stage, which is required for normal muscle development and normal defecation and egg-laying. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf HLH-8 was tagged with a dual GFP:3xFLAG tag at the carboxy terminus, whose expression pattern was confirmed through both fluorescence imaging and immunoblot analysis. The binding sites were determ

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Michael Wilson 

PROVIDER: E-GEOD-17458 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress