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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development

ABSTRACT: The roles of histone demethylase KDM7 in gene expression were analyzed by gene expression profiling experiments with mouse neuroblastoma cell line Neuro2A. Keywords: mouse neuroblastoma, Neuro2A, gene expression profiling, microarray, Affimetrix M430 2.0 chip In order to examine the effect of KDM7 in gene expression, we generated stable KDM7 knockdown cell lines in mouse neuroblastoma cell line Neuro2A. Total RNAs were extracted from 5 cell lines (parental cells: Neuro2A, empty vector: Neuro2A transfected with empty vector, EGFP KD: Neuro2A transfected with vector for EGFP knock down, KDM7 KD1: Neuro2A transfected with vector 1 for KDM7 knock down, and KDM7 KD2: Neuro2A transfected with vector 2 for KDM7 knock down) and analyzed for gene expression profiles using Affymetrix platform.

ORGANISM(S): Mus musculus

SUBMITTER: Yuichi Tsukada

PROVIDER: E-GEOD-17494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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