Transcription profiling of E. coli high-cell-density recombinant and wild-type

ABSTRACT: The transcriptome profiles for wild-type (plasmid-free) and recombinant (plasmid-bearing) Escherichia coli during well-controlled synchronized high-cell-density fed-batch cultures were analyzed by DNA microarrays. It was observed that the growth phase significantly affected the transcriptome profiles, and the transcriptome profiles were significantly different for the recombinant and wild-type cultures. The response of the wild-type and recombinant cultures to an isopropyl-1-thio-β-D-galactopyranoside- (IPTG-) addition was examined, where IPTG induced recombinant protein production in the plasmid-bearing cultures. The IPTG-addition significantly altered the transcriptome response of the wild-type cultures entering the stationary phase. The IPTG-induced recombinant protein production result

ORGANISM(S): Escherichia coli

SUBMITTER: Sarah Harcum 

PROVIDER: E-GEOD-17505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress