Transcription profiling of E. coli MG1655 treated with serine hydroxamate

ABSTRACT: For over 30 years, serine hydroxamate has been used to chemically stimulate a stringent response in Escherichia coli and other bacteria. These studies have elucidated numerous characteristics of the classical stringent response beyond the simple cellular response to an amino acid shortage, including phospholipid synthesis and protease up-regulation. In this study, the effects of a serine hydroxamate addition on high cell density recombinant E. coli were examined and compared to the effects of recombinant protein production to determine overlaps, as recombinant protein production stress has often been attributed to amino acid shortages. Both the transcriptome and growth characteristics were evaluated and compared. The serine hydroxamate addition profoundly decreased the culture growth rate

ORGANISM(S): Escherichia coli

SUBMITTER: Sarah Harcum 

PROVIDER: E-GEOD-17506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress