Transcription profiling of recombinant E. coli to heat-shock and dual heat-shock recombinant protein induction

ABSTRACT: Recombinant Escherichia coli cultures are used to manufacture numerous therapeutic proteins and industrial enzymes, where many of these processes use elevated temperatures to induce recombinant protein production. The heat-shock response in wild-type E. coli has been well studied. In this study, the transcriptome profiles of recombinant E. coli subjected to a heat-shock and to a dual heat-shock recombinant protein induction were examined. Most classical heat-shock protein genes were identified as regulated in both conditions. The major transcriptome differences between the recombinant and reported wild-type cultures were heavily populated by hypothetical and putative genes, which indicates recombinant cultures utilize many unique genes to respond to a heat-shock. Comparison of the dua

ORGANISM(S): Escherichia coli

SUBMITTER: Sarah Harcum 

PROVIDER: E-GEOD-17507 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress