Features of cryptic promoters and their varied reliance on bromodomain-containing factors

ABSTRACT: The Set2-Rpd3S pathway is important for the control of transcription memory. Mutation of components of this pathway results in cryptic transcription initiation within the coding region of approximately 30% of yeast genes. Specifically, deletion of the Set2 histone methyltransferase or Rco1, a component of the Rpd3S histone deacetylase complex leads to hyperacetylation of certain open reading frames (ORFs). We used this mutant as a system to study the role of histone modifications and co-activator recruitment in preinitiation complex (PIC) formation. Specifically, we looked at the dependence of promoters on the bromodomain-containing RSC complex and the Bdf1 protein. We found that the dependence of cryptic promoters for these proteins varied. Overall, our data indicates that cryptic promot

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Samantha Pattenden 

PROVIDER: E-GEOD-17521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress