Global comparison of mouse ES cell lines derived from blastocysts and preblastocysts.

ABSTRACT: Standard mouse ES cell derivation protocols use blastocysts at 3.5 dpc, but the efficiency of successful line derivation is low in commonly-used inbred strains, and much lower in other mouse strains. By using 2-cell zona pellucida-free embryos, culturing them on embryonic feeder cells, and passaging the outgrowths in several different media formulations, we have dramatically improved mouse ES cell line derivation efficiency. Mouse ES cell lines derived from blastocyst outgrowths using standard procedures were compared to preblastocyst (2-cell outgrowth)-derived lines, each derived in three different media formulations, using the genome-scale MEEBO gene expression oligo probe set. The overall goal of this microarray study is to determine the overall similarity between cell lines derived usi

ORGANISM(S): Mus musculus

SUBMITTER: Mark Carter 

PROVIDER: E-GEOD-17560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress