Inactivation of Unr results in induction of differentiation of murine ES cells into the primitive endoderm lineage

ABSTRACT: Unr (upstream of N-ras) is a cytoplasmic RNA-binding protein with cold shock domains, involved in regulation of messenger RNA stability and translation. To address the biological role of Unr, we inactivated the unr gene by homologous recombination in mice and embryonic stem (ES) cells. Embryos deficient for Unr die at mid-gestation, and the main phenotypic defects observed, growth deficiency and absence of neural tube closure, suggest a role of Unr in the balance proliferation/differentiation during early development. Here, we report that in Unr-null ES cell cultures, we observed a greater proportion of partially differentiated colonies, together with dispersed, refractile cells with stellate morphology, reminiscent of primitive endoderm (PrE) cells. DNA microarray, immunostaining, and RN

ORGANISM(S): Mus musculus

SUBMITTER: Doulaye Dembele 

PROVIDER: E-GEOD-17566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress