Comparative global transcriptomic profiling of human ES and iPSs cells and their derived microdissected cardiac clusters

ABSTRACT: Recent establishment of induced pluripotent stem (iPS) cells opened new avenues for generating human patient-specific stem cell derivatives that can be used for in vitro modeling of human disease, drug development and cell replacement therapy. In this study we analyzed the molecular and functional properties of cardiomyocytes (CM) differentiated from human iPS cells. Clusters of synchronously beating cells were first observed at day 11 of iPS cell differentiation. Beating clusters that were microdissected at day 18 of differentiation expressed high levels of cardiospecific transcripts NKx2.5, alpha-MHC, MLC2v, alpha-actinin and troponin T. Immunocytochemical stainings for alpha-actinin and troponin T revealed cross-striations typical of CM. Functional assessment of iPS cell-derived CM sho

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Gaarz 

PROVIDER: E-GEOD-17579 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress