Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in response to AGL15 during somatic embryogenesis

ABSTRACT: Transcript accumulation was measured using the Affymetrix Arabidopsis ATH1 Genome Array [ATH1-121501] to document changes in response to the MADS-domain transcription factor AGAMOUS-Like 15 during somatic embryogenesis. A somatic embryo system was used where mature seed is allowed to complete germination in liquid MS media containing 2,4-D and seedlings produce somatic embryos from the shoot apical meristem (SAM) region. The frequency with which these embryos are produced directly correlates with AGL15 accumulation. Experiment Overall Design: Wild type Arabidopsis (Columbia ecotype) with normal amounts of AGL15 were compared to (1) 35S:AGL15 that constitutively expresses AGL15 and produces more somatic embryos from the shoot apical region than wild type and (2) agl15 agl18 double loss-of-function mutants that produce less somatic embryos from the shoot apical region than wild type.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sharyn Perry 

PROVIDER: E-GEOD-17610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Global identification of targets of the Arabidopsis MADS domain protein AGAMOUS-Like15.

Zheng Yumei Y   Ren Na N   Wang Huai H   Stromberg Arnold J AJ   Perry Sharyn E SE  

The Plant cell 20090918 9

AGAMOUS-Like15 (AGL15) is a MADS domain transcriptional regulator that promotes somatic embryogenesis by binding DNA and regulating gene expression. Chromatin immunoprecipitation (ChIP) analysis previously identified DNA fragments with which AGL15 associates in vivo, and a low-throughput approach revealed a role for AGL15 in gibberellic acid catabolism that is relevant to embryogenesis. However, higher throughput methods are needed to identify targets of AGL15. Here, we mapped AGL15 in vivo bind  ...[more]

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