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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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A map of open chromatin in human pancreatic islets

ABSTRACT: High-throughput sequencing of genomic regions isolated using FAIRE (Formaldehyde-assisted isolation of regulatory elements) from three purified pancreatic islet samples For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Identification of nucleosome-depleted genomic regions in three human pancreatic islet samples This work was carried out in the laboratories of Jason Lieb and Jorge Ferrer, and was supported in part by a subcontract to J. Lieb from Greg Crawford's ENCODE grant.

ORGANISM(S): Homo sapiens

SUBMITTER: Kyle Gaulton

PROVIDER: E-GEOD-17616 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A map of open chromatin in human pancreatic islets.

Gaulton Kyle J KJ Nammo Takao T Pasquali Lorenzo L Simon Jeremy M JM Giresi Paul G PG Fogarty Marie P MP Panhuis Tami M TM Mieczkowski Piotr P Secchi Antonio A Bosco Domenico D Berney Thierry T Montanya Eduard E Mohlke Karen L KL Lieb Jason D JD Ferrer Jorge J

Nature genetics 20100131 3

Tissue-specific transcriptional regulation is central to human disease. To identify regulatory DNA active in human pancreatic islets, we profiled chromatin by formaldehyde-assisted isolation of regulatory elements coupled with high-throughput sequencing (FAIRE-seq). We identified approximately 80,000 open chromatin sites. Comparison of FAIRE-seq data from islets to that from five non-islet cell lines revealed approximately 3,300 physically linked clusters of islet-selective open chromatin sites, ...[more]

PMID: 20118932

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