Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Experiment 4: Affymetrix validation array

ABSTRACT: Zebrafish embryo were analyzed at 30 and 60 % epiboly for changes in transcriptome of wild-type and MTspg mutant embryos The Data are used as an independent experimental validation of data obtained for the same stages by Agilent arrays in the main experiments (especially Exp. 1) Experiments were performed in biological duplicate. Genedata Expressionist Analyst was used for comparison betwen MZspg and WT gene expression levels at each stage

ORGANISM(S): Danio rerio

SUBMITTER: Daria Onichtchouk 

PROVIDER: E-GEOD-17657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Zebrafish Pou5f1-dependent transcriptional networks in temporal control of early development.

Onichtchouk Daria D   Geier Florian F   Polok Bozena B   Messerschmidt Daniel M DM   Mössner Rebecca R   Wendik Björn B   Song Sungmin S   Taylor Verdon V   Timmer Jens J   Driever Wolfgang W  

Molecular systems biology 20100309

The transcription factor POU5f1/OCT4 controls pluripotency in mammalian ES cells, but little is known about its functions in the early embryo. We used time-resolved transcriptome analysis of zebrafish pou5f1 MZspg mutant embryos to identify genes regulated by Pou5f1. Comparison to mammalian systems defines evolutionary conserved Pou5f1 targets. Time-series data reveal many Pou5f1 targets with delayed or advanced onset of expression. We identify two Pou5f1-dependent mechanisms controlling develop  ...[more]

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