Transcriptomic analysis comparing the spontaneous acapsular strain AL1114 to the parent capsulated strain AL1115

ABSTRACT: P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida fowl cholera-causing strains but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. Quantitative RT-PCR showed that these strains had significantly reduced transcription of the capsule biosynthetic genes, but DNA sequence analysis identified no mutations within the cap biosynthetic locus. However, whole genome sequencing of paired capsulated and acapsular strains ident

ORGANISM(S): Pasteurella multocida

SUBMITTER: Jason Steen 

PROVIDER: E-GEOD-17686 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress