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Luteinisation genes

ABSTRACT: Two SAGE libraries generated before (PMSG) or after (PMSG/hCG) the onset of granulosa cell luteinisation induced by hCG Keywords = granulosa Keywords = PMSG Keywords = hCG Keywords = luteinisation Keywords: other

ORGANISM(S): Mus musculus

SUBMITTER: Peter O'Shaughnessy

PROVIDER: E-GEOD-1771 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Changes in mouse granulosa cell gene expression during early luteinization.

McRae R S RS Johnston H M HM Mihm M M O'Shaughnessy P J PJ

Endocrinology 20040930 1

Changes in gene expression during granulosa cell luteinization have been measured using serial analysis of gene expression (SAGE). Immature normal mice were treated with pregnant mare serum gonadotropin (PMSG) or PMSG followed, 48 h later, by human chorionic gonadotropin (hCG). Granulosa cells were collected from preovulatory follicles after PMSG injection or PMSG/hCG injection and SAGE libraries generated from the isolated mRNA. The combined libraries contained 105,224 tags representing 40,248 ...[more]

PMID: 15459113

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Luteinisation genes

Project description:Two SAGE libraries generated before (PMSG) or after (PMSG/hCG) the onset of granulosa cell luteinisation induced by hCG Keywords = granulosa Keywords = PMSG Keywords = hCG Keywords = luteinisation Keywords: other

2004-09-17 | GSE1771 | GEO

Granulosa cell gene expression profiling of bovine cohort, dominant and subordinate follicles

Project description:Bovine granulosa cells harvested from cohort follicles at 1.3 days after wave emergence, and from dominant and subordinate follicles at 2.6 days after wave emergence. Total RNA extracted from granulosa cells of 4 cohort, 4 dominant and 4 subordinate follicles was pooled for generation of each respective SAGE library. Keywords: other

2005-04-30 | E-GEOD-2598 | biostudies-arrayexpress

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eye-SAGE

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Detecting novel low-abundant transcripts in Drosophila

Project description:Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244,313 SAGE tags from transcripts expressed in Drosophila embryonic, larval, pupae, adult, and testicular tissue. From these SAGE tags, we identified 40,823 unique SAGE tags. Our analysis showed that 55% of the 40,823 unique SAGE tags are novel without matches in currently known Drosophila transcripts, and most of the novel SAGE tags have low copy numbers. Further analysis indicated that these novel SAGE tags represent novel low-abundant transcripts expressed from loci outside of currently annotated exons including the intergenic and intronic regions, and antisense of the currently annotated exons in the Drosophila genome. Our study reveals the presence of a significant number of novel low-abundant transcripts in Drosophila, and highlights the need to isolate these novel low-abundant transcripts for further biological studies. Keywords: other

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C. elegans SAGE

Project description:The SAGE libraries were built from total mRNA populations in specific tissues, cells, mutation-specific populations, and all developmental stages of C. elegans. Tissue- and cell-specific libraries were generated from FACS-sorted cells marked by expression of specific promoter::GFP fusions Keywords = SAGE Keywords = LongSAGE Keywords = Caenorhabditis elegans Keywords = development Keywords = dauer Keywords: other

2004-06-11 | E-GEOD-1447 | biostudies-arrayexpress

Control vs. AngII Infusion: Heart

Project description:C57Bl/6J mice received continuous administration of saline or Angiotensin II via implanted minipumps for 7 days, total RNA prepared from homogenized hearts, SAGE library constructed from heart RNA pooled from 6 mice. Keywords: other

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Adult mouse testis

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Project description:SAGE identification of differentiation responsive genes in P19 embryonic cells induced to form cardiomyocytes in vitro. P19 embryonic carcinoma (EC) cells, induced to form cardiomyocytes in vitro - undifferentiated cells, day 3+0.5 and day 3+3.0 of differentiation protocol. Keywords = EC cells, P19, differentiation, cardiomyocytes Keywords: time-course

2002-07-18 | E-GEOD-42 | biostudies-arrayexpress

gene expression profiles of Barrett's esophagus, squamous esophagus and gastric cardia

Project description:SAGE performed on biopsies of Barrett's esophagus, squamous esophagus and gastric cardia taken from a metaplastic Barrett's esophagus patient. Keywords: SAGE comparative analysis of gene expression profiles of Barrett's esophagus, normal squamous esophagus and gastric cardia tissue

2005-06-20 | E-GEOD-2769 | biostudies-arrayexpress

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