Ecdysone signaling and transcript signature in Drosophila cells resistant against methoxyfenozide

ABSTRACT: Methoxyfenozide (RH-2485) represents the group of non-steroidal ecdysteroid agonists with a dibenzoylhydrazine structure, that are used as novel biorational insecticides in the control of insect pests. Here we report on the selection of Drosophila melanogaster S2 cells for resistance to inhibition of cell proliferation by methoxyfenozide by ~1000-fold over 4 months. Cells were exposed to gradually increasing concentrations of methoxyfenozide and selected out based on the ecdysteroid-sensitive response for cell proliferation. In the resistant cells, the ecdysteroid receptor (EcR/USP) complex was no longer active in the presence of methoxyfenozide. But when resistant cells were relaxed from pressure in methoxyfenozide-free medium, induction of the reporter construct was observed. In parallel

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Guy Smagghe 

PROVIDER: E-GEOD-17748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress