Biallelic and complicated inactivation of Pten in radiation-induced mouse thymic lymphomas

ABSTRACT: Inactivation of Pten occurs by multiple mechanisms including epigenetic silencing, point mutations, insertion, and deletion, which are tissue dependent. Although frequent loss of heterozygosity around Pten locus and plausible involvement of epigenetic silencing have been reported in radiation-induced thymic lymphomas, the frequency of Pten inactivation and the spectrum of causal aberrations have not yet been extensively characterized. Here, we assessed the principal mode of inactivation by comprehensively analyzing expression and alterations of Pten gene in 23 radiation-induced thymic lymphomas developed in B6C3F1 mice. We found no evidence for methylation-associated silencing of Pten gene. Instead, we found complex structural abnormalities in 8 lymphomas (35%) that included missense and n

ORGANISM(S): Mus musculus

SUBMITTER: Yoshiya Shimada 

PROVIDER: E-GEOD-17751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress