Impact of RNA degradation on gene expression profiling

ABSTRACT: 1.) Background: Gene expression profiling is a highly sensitive technique which is used for profiling of tumor samples for medical prognosis. RNA quality and degradation influences the analysis results of gene expression profiles. The impact of this influence on the profiles and its medical impact is not fully understood. As patient samples are very valuable for clinical studies, it is necessary to establish criteria for the RNA quality to use these samples in later analysis. 2.) Methods: To investigate the effects of RNA integrity on gene expression profiling whole genome expression arrays were used. We therefore used tumor biopsies from patients diagnosed with locally advanced rectal cancer. To simulate degradation, the isolated total RNA of all patients was subjected to heat-induced deg

ORGANISM(S): Homo sapiens

SUBMITTER: Lennart Opitz 

PROVIDER: E-GEOD-17753 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress