Transcription profiling of human mesenchymal stem cells with and without TGF-B vs human annulus disc cells

ABSTRACT: Autologous stem cell therapy has potential for biologic treatment of disc degeneration. Due to ease of harvest and abundance, adipose-derived mesenchymal stem cells (AD-MSC) are readily available. Our objectives were: 1) To develop/validate methods to harvest AD-MSC and direct them to a disc-like phenotype by three-dimensional (3D) culture and TGF-ß3 exposure; 2) To perform gene expression profiling for human AD-MSC and annulus cells in 3D culture; 3) To test whether disc cell-AD-MSC co-culture could augment proteoglycan production. Stem cell plasticity offers potential for future biologic therapies for disc degeneration. Data indicated that human AD-MSC can successfully be manipulated in 3D culture to express gene products important in the disc ECM (types I and II collagen, chondroiti

ORGANISM(S): Homo sapiens

SUBMITTER: Helen Gruber 

PROVIDER: E-GEOD-17782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress