Tiling microarray-based identification of small regulatory RNAs in the group A Streptococcus (GAS)

ABSTRACT: Small regulatory RNAs (sRNAs) represent a major mechanism of virulence regulation in several pathogens. To date, only three sRNAs have been described in GAS in any detail. To identify whether sRNAs are common within the GAS genome, we created a custom Affymetrix tiling microarray in which all intergenic regions (>49 bp) of the MGAS2221 genome were tiled at high density. Triplicate cultures of strain MGAS2221 in THY broth were grown to the exponential phase (O.D.600 of 0.5) of growth and RNA was isolated, converted to cDNA, fragmented, labeled, and hybridized to our custom array (one array per RNA sample [ hence three arrays total]). Our data identified the presence of 40 candidate sRNAs within the MGAS2221 genome. Three cultures of MGAS2221 were grown in THY broth to the exponential (O

ORGANISM(S): Streptococcus pyogenes

SUBMITTER: Paul Sumby 

PROVIDER: E-GEOD-17789 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress