The comparison of CpG methylation profiles between CD34+ and CD34- human umbilical cord blood cells

ABSTRACT: Evidence for the epigenetic regulation of hematopoietic stem cells (HSCs) is growing, but the genome-wide epigenetic signature of HSCs and its functional significance remain unclear. In this study, we used high-resolition (Agilent, CpG Island ChIP-on-chip) platforms for genome-wide comparison of CpG methylation in human CD34+ and CD34- cells. CD34+ and CD34- cells were purified from human umbilical cord blood. Genomic DNA obtained from CD34+ and CD34- cells was fragmented by either sonication or digestion with MseI. Recombinant methyl-binding domain (Genomictree, Daejeon, Korea) was incubated with sonicated genomic DNA in a binding reaction mixture. The methyl-enriched DNA fraction was purified using Qiaquick PCR purification kits (Qiagen, Hilden, Germany) and amplified using a whole gen

ORGANISM(S): Homo sapiens

SUBMITTER: Il-Hoan Oh 

PROVIDER: E-GEOD-17833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress