Mice with FIA develop anti-native and anti-in vitro citrullinated fibrinogen antibodies

ABSTRACT: Synovial antigen arrays were probed with 1:150 dilutions of plasma derived from SJL mice immunized with fibrinogen emulsified in CFA or with CFA alone. Autoantibody binding was detected with a Cy3-conjugated goat-anti-mouse IgG/M secondary antibody. SAM was applied to identify antigens with statistically significant differences in array reactivity between FIA and CFA control plasma (q < 0.01) obtained from mice before boosting. The SAM hits were subjected to hierarchical cluster analysis and are displayed as a heatmap. Synovial array profiling of FIA plasma demonstrated autoreactive B-cell responses against peptides representing native fibrinogen, and B-cell epitope spreading resulting in additional targeting of citrullinated fibrinogen in the samples obtained before boosting. Custom-s

ORGANISM(S): Mus musculus

SUBMITTER: Ricardo Paniagua 

PROVIDER: E-GEOD-18023 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress